A paleodietary analysis of the mid-Holocene mortuary site, Windover (8BR246), based on carbon and nitrogen bone-collagen values and archaeobotanical information is consistent with a subsistence strategy that utilized river-dwelling fauna and a range of terrestrial flora, such as grapes and prickly pear. The isotopic analysis does not support the extensive human dietary use of either marine mammals or classic terrestrial fauna such as deer or rabbit. Seasonal (late summer/early fall) use of the site is indicated by the range of flora found in association with the burials.